Beekeeping at FiveApple

Ep161 Making sense of the assays—with Ang Roell of They Keep Bees

Beekeeping at FiveApple

Show Notes

UBO, PKB, FKB, mite washes, Harbo assays... It's an alphabet soup out there of ways to test your bees for hygienic traits and resistance to varroa. You may be testing to find out when to apply mite treatments or a brood break to a colony and in that case a simple mite wash is usually enough. But you may be testing to find out your very best genetics you have to raise next years queens for your yards and in that case, you probably need need more information than a mite wash provides. Or do you? Do these assays work, how reliably do they work and which one is the most reliable, if any? Ang Roell and Bi Kline of They Keep Bees are working to find out with a new NE SARE grant comparison study. Today Ang and I talk about the cornucopia of testing methods that each have their complications and limitations. We'll also find out what Ang and Bi will explore in this ongoing study. Plus, we chat bees, of course. :-) Hope you enjoy this weeks episode! kind regards, Leigh

The Study (read the full grant proposal and study design here) In 2025 national honey bee losses exceeded 60% in commercial beekeeping operations. Miticide resistance was discovered in 100% of tested samples, highlighting an urgent need for biology-based strategies that reduce chemical dependence while strengthening colony resilience. This project evaluates the reliability and heritability of four major hygienic behavior assays, in order to determine which methods best predict mite resistance and overwinter survival. We will compare assay performance in twelve breeder colonies (six selected, six controls) and their untreated F1 daughters. Across two seasons, all colonies will undergo standardized rounds of UBO, PKB, and FKB testing, as well as mite washes and Harbo assays. By analyzing correlations among assay scores, mite loads, reproductive mite presence, and overwinter survival, we will identify which tools reliably capture heritable Varroa-resistant traits and which factors influence assay consistency. This project addresses a documented need for effective selection tools that help beekeepers identify and propagate Varroa-resistant stock. Results will be shared through a report, digital outreach, and a presentation at Cornell's Dyce Lab. A hands-on workshop at They Keep Bees will train beekeepers to practice each assay method. By providing evidence-based guidance to at least 100 beekeepers, the project aims to enable measurable improvements such as higher overwinter survival and reduced reliance on miticides. Project objectives from proposal: Primary Research Questions

Do pin-killed brood (PKB), freeze-killed brood using liquid nitrogen (FKB), and unhealthy brood odor (UBO) assays provide predictive measures of heritable mite-resistant traits in F1 daughter queens?

Are these assays also predictive of overall colony mite loads and winter survival outcomes? Winter survival will be defined as survival from August of year 1 until March of year 2.

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More from Beekeeping at FiveApple

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Oct 1, 2026

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Sep 17, 2026

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[Last Thursday Apple podcasts had an outage and the episode didn't show up as a new one in your feed. Here it is again for the Apple folks -- just a duplicate of last weeks episode that was missing for many! --Leigh] Listeners, This has been a test year in my bee yards, to see if my old treatment-free methods that worked so well for 16 years in an isolated bee yard would hold up in areas with more beekeepers. Answer: Nope. [Deep sigh and regroup. ] I now feel so lucky to have learned a hybrid system that still allows me to work on my long term project of stock selection towards mite resistance–while also utilizing organic acids for mite clean up in colonies that don't make the cut, with a requeen afterwards with the better genetics. Back on track! Thank you to queen breeders Ryan Williamson, Ang Roell, Troy Hall , James Lee and YouTube legend Bob Binnie for helping me understand a system that allows for both. Thank you all so much for pioneering a method that gave me resolve to farmer up! I'm happy to have a plan and have put it underway. in gratitude, Leigh Thank you to the supporting Patrons. You make this podcast possible as well as keeping all of us from needing to listen to adverts! Thank you to Lisa N the newest podcast angel and thank you to Bev M, Anderson M, Greg K, Dan G, Donald F, and Kerri A for joining this community this summer! Welcome to you all! And a special shout out to podcast angels Michael C and Will C, plus Jeff T, Deborah P, Chuck J, Bruce H and Blaine N for being with the podcast since it started in 2019! What a huge amount of gratitude I have for your continued support. It's thanks to you this podcast still exists.

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